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You are here:Home » Molecular Biology » Growth Factors-Cell Factors » Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth
Factor, MGF)

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Specifications

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.3kD polypeptide containing 164aa, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all non-animal reagents.
Catalog #149761
SourceRecombinant protein corresponding to mouse SCF, expressed in E.coli.
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The ED50 was found to be 10ng/ml.
Specific Activity1x10e7units/mg
Endotoxin<0.1ng/ug (1EU/ug)
AA SequenceMKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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