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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Adrenomedullin (ADM) Recombinant, Human

Adrenomedullin (ADM) Recombinant, Human

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Specifications

Recombinant protein corresponding to human ADM with a N-terminal His tag expressed in E. coli (P35318).
Catalog #153390
Purity 95%
AA SequenceMGHHHHHHSGSEF-ARLDVASEF RKKWNKWALS RGKRELRMSS SYPTGLADVK AGPAQTLIRP QDMKGASRSP EDSSPDAARI RVKRYRQSMN NFQGLRSFGC RFGTCTVQKL AHQIYQFTDK DKDNVAPRSK ISPQGYG
Predicted Isoelectric Point10.3
Predicted Molecular Weight~15.8kD
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight15.8kD
SourceE. coli
Purity95% Endotoxin: 1.0EU per 1ug (LAL)
FormSupplied as a lyophilized powder in PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.0.1% trehalose, 0.0.1% sodium azide. Reconstitute in sterile PBS, pH 7.3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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