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You are here:Home » Molecular Biology » Growth Factors-Fetuin » Alpha-2-Heremans Schmid Glycoprotein (aHSG, Alpha-2-HS-Glycoprotein, A2HS, AHS, FETUA) Recombinant, Human

Alpha-2-Heremans Schmid Glycoprotein (aHSG, Alpha-2-HS-Glycoprotein, A2HS, AHS, FETUA)
Recombinant, Human

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Specifications

AHSG, also known as fetuin-A, belongs to the fetuin class of plasma binding proteins. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. It is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has been postulated that it participates in the development of the tissues. It forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate.
Catalog #153448
Alpha2-HS glycoprotein, a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.[3]
The choroid plexus is an established extrahepatic expression site. The mature circulating AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. Multiple post-translational modifications have been reported.[4] Thus AHSG is a secreted partially phosphorylated glycoprotein with complex proeolytic processing that circulates in blood and extracellular fluids. In the test tube AHSG can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Most of these functions await confirmation in vivo.
Fetuins like histidine-rich glycoprotein and kininogens are secreted (so-called type 3) cystatins.[5] Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate.[6][7] Thus fetuin-A is a potent inhibitor of pathological calcification. Mice deficient in fetuin-A show systemic calcification of soft tissues.[8][9]
SourceRecombinant Human from E. coli
Purity 95%
Endotoxin1.0EU per 1ug (determined by the LAL method)
Accession NoP02765
FragmentAla19~Leu300 (Accession No: P02765)
SequenceMSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- AP HGPGLIYRQP NCDDPETEEA ALVAIDYINQ NLPWGYKHTL NQIDEVKVWP QQPSGELFEI EIDTLETTCH VLDPTPVARC SVRQLKEHAV EGDCDFQLLK LDGKFSVVYA KCDSSPDSAE DVRKVCQDCP LLAPLNDTRV VHAAKAALAA FNAQNNGSNF QLEEISRAQL VPLPPSTYVE FTVSGTDCVA KEATEAAKCN LLAEKQYGFC KATLSEKLGG AEVAVTCTVF QTQPVTSQPQ PEGANEAVPT PVVDPDAPPS PPLGAPGLPP AGSPPDSHVL
Epitope TagN-terminal Tags: His-tag and GST-tag
Molecular Weight62.2kD
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight62.2kD
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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