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You are here:Home » Molecular Biology » Growth Factors-Fetuin » Alpha-2-Heremans Schmid Glycoprotein (aHSG, Alpha-2-HS-Glycoprotein, A2HS, AHS, FETUA) Recombinant, Rat

Alpha-2-Heremans Schmid Glycoprotein (aHSG, Alpha-2-HS-Glycoprotein, A2HS, AHS, FETUA)
Recombinant, Rat

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Specifications

AHSG, also known as fetuin-A, belongs to the fetuin class of plasma binding proteins. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. It is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has been postulated that it participates in the development of the tissues. It forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate.
Catalog #153450
Alpha2-HS glycoprotein, a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.[3]
The choroid plexus is an established extrahepatic expression site. The mature circulating AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. Multiple post-translational modifications have been reported.[4] Thus AHSG is a secreted partially phosphorylated glycoprotein with complex proeolytic processing that circulates in blood and extracellular fluids. In the test tube AHSG can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Most of these functions await confirmation in vivo.
Fetuins like histidine-rich glycoprotein and kininogens are secreted (so-called type 3) cystatins.[5] Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate.[6][7] Thus fetuin-A is a potent inhibitor of pathological calcification. Mice deficient in fetuin-A show systemic calcification of soft tissues.[8][9]
SourceRecombinant Rat from E. coli
Purity 95%
Endotoxin1.0EU per 1ug (determined by the LAL method)
Accession NoP24090
FragmentAla19~Phe250 (Accession No: P24090)
SequenceMGHHHHHHSGSEF-AP QGAGLGFREL ACDDPETEHV ALIAVDYLNK HLLQGFRQIL NQIDKVKVWS RRPFGEVYEL EIDTLETTCH ALDPTPLANC SVRQQAEHAV EGDCDFHILKQDGQFRVLHA QCHSTPDSAE DVRKFCPRCP ILIRFNDTNV VHTVKTALAA FNAQNNGTYF KLVEISRAQN VPFPVSTLVE FVIAATDCTG QEVTDPAKCN LLAEKQYGFC KATLIHRLGG EEVSVACKLF
Epitope TagN-terminal His-Tag
Molecular Weight27.3kD
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.
Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight27.3kD
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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