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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Anti-Mullerian Hormone (AMH) Recombinant, Human

Anti-Mullerian Hormone (AMH) Recombinant, Human

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Specifications

Recombinant protein corresponding to aa453-560 with a N-Terminal HGis-Tag from human AMH expressed in E. coli (P03971)
Catalog #153545
Amino Acid SequenceMGHHHHHHSGSEF- AGATAADG PCALRELSVD LRAERSVLIP ETYQANNCQG VCGWPQSDRN PRYGNHVVLL LKMQVRGAAL ARPPCCVPTA YAGKLLISLS EERISAHHVP NMVATECGCR
Predicted Molecular Weight13.1kD
Predicted Isoelectric Point7.7
Subcellular LocationSecreted
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and StabilityLyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight13.1kD
SourceE. coli
Purity95%. Endotoxin: 1EU/ug (LAL)
FormSupplied as lyophilized powder from PBS, pH 7.4, 5% sucrose, 0.01% sarcosyl. Reconstitute with sterile PBS, pH 7.3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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