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You are here:Home » Molecular Biology » Growth Factors-CTGF » Connective Tissue Growth Factor (CTGF) Recombinant, Human

Connective Tissue Growth Factor (CTGF) Recombinant, Human

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Specifications

Recombinant protein corresponding to aa80~200 (P29279), with a N-terminal Tag, His-tag expressed in E. coli.
Catalog #154171
Amino Acid SequenceMGHHHHHHSGSEF-L FCHFGSPANR KIGVCTAKDG APCIFGGTVY RSGESFQSSC KYQCTCLDGA VGCMPLCSMD VRLPSPDCPF PRRVKLPGKC CEEWVCDEPK DQTVVGPALA AYRLEDTFGP DPTMIRANCL
Predicted Molecular Weight14.6kD
Subcellular LocationSecreted, extracellular space, extracellular matrix
Predicted isoelectric point6.6
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7. 1mM EDTA, 2% SDS, 100mM DTT, 1m sodium azide, 0.01% bromo-phenol blue, 33% glycerol.
Add 3-5ul/well for mini gels; 7ul/well for large gels.
Store at -20 Stable for 6 months after receipt.
NoteThermal stability is described by the los rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Storage and StabilityLyophilized powder may be stored at -70°C. Stable for 12 months at -70°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS NumberN/A
Molecular Weight14.6kD
SourceE. coli
Purity~95% Endotoxin: 1EU/ug (LAL)
FormSupplied as a lyophilized powder from PBS, pH7.4, 1mmM DTT, 5% trehalose, 0.05% sarcosyl, 0.02% sodium azide. Reconstitute with PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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