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You are here:Home » Molecular Biology » Growth Factors-GFAP » Glial Fibrillary Acidic Protein (GFAP) Recombinant, Human

Glial Fibrillary Acidic Protein (GFAP) Recombinant, Human

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Specifications

Recombinant protein corresponding to aa254-374 with two N-terminal Tags, His-Tag and S-Tag from human GFAP, expressed in E. coli (P14136)
Catalog #154757
Amino Acid SequenceMHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-EEWYRSKF ADLTDAAARN AELLRQAKHE ANDYRRQLQS LTCDLESLRG TNESLERQMR EQEERHVREA ASYQEALARL EEEGQSLKDE MARHLQEYQD LLNVKLALDI EIATYRKLLE GEE
Predicted Molecular Weight20kD
Accurate Molecular Weight25kD as determined by SDS-PAGE reducing conditions
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene
2. Relative charge: The composition of amino acids may affect the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Predicted Isoelectric Point5.1
Subcellular LocationCytoplasm
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight20.0kD
SourceE. coli
Purity95%. Endotoxin: 1EU/ug (LAL)
FormSupplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.02% sodium azide. Reconstitute with sterile ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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