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You are here:Home » Molecular Biology » MB-Growth Factors-Interferon » Interferon alpha (IFNa) Recombinant, Rabbit

Interferon alpha (IFNa) Recombinant, Rabbit

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Specifications

Recombinant protein corresponding to aa1-93 with two N-Terminal Tags, His-Tag and GST-Tag from rabbit IFNa, expressed in E. coli (Q75T05)
Catalog #155258
Amino Acid SequenceMRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF- SCLKDRKDFG FPLEKVDAQQ IQKAQAISIL HELSQQVLNI YTSXDSSAAW DATLLDSFCN DLQQQLSGLQ ACQMHQVGVQ EPPLAQEDSL LAV
Predicted Molecular Weight40.3kD
Predicted Isoelectric Point4.4
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Unstained Protein Molecular Weight Marker, 10-70kD
Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.
Protein Bands 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
Usage Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:
Mini gels: 3-5ul/well
Large gel: 7ul/well
The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.
Store at -20°C Stable for 6 months after receipt.
Storage and StabilityLyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight40.3kD
SourceE. coli
Purity95%; Endotoxin: 1EU/ug
FormSupplied as lyophilized powder from PBS, pH 7.4, 0.05% sarcosyl. Reconstitute with sterile PBS, pH 7.3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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