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You are here:Home » Molecular Biology » MB-Neurotrophins » Neurotrophin 3 (NT3) Recombinant, Human

Neurotrophin 3 (NT3) Recombinant, Human

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Specifications

Recombinant protein corresponding to aa21-138 with a N-terminal His-Tag from human NT3, expressed in E. coli (P20783)
Catalog #156083
Predicted Molecular Weight15kD
Predicted Isoelectric Point9.3
Subcellular LocationSecreted
Amino Acid SequenceMGHHHHHHSGSEF-MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM EDYVGSPVVA NRTSRRKR
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
NoteThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight15kD
SourceE. coli
Purity95%. Endotoxin: 1EU/ug (LAL)
FormSupplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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