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You are here:Home » Kits and Assays » Kits for ELISA (Hormones & Steroids), BioAssay™ » 17-alpha hydroxy Progesterone BioAssay™ ELISA Kit Not for use in USA, for Export use only

17-alpha hydroxy Progesterone BioAssay™ ELISA Kit Not for use in USA, for Export use only

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Specifications

17-alpha hydroxy Progesterone BioAssay™ ELISA Kit is a C-21 steroid hormone produced in the adrenal gland and gonads, during the synthesis of glucocorticoids and sex steroids. It is derived from progesterone via 17-hydroxylase, a P450c17 enzyme, or from 17-hydroxypregnenolone via 3ß-hydroxysteroid dehydrogenase/?5-4 isomerase. 17a -OHP has no defined physiologic role except as a precursor molecule. Serum 17a -OHP levels are age-dependent, with peak levels observed during fetal life and the immediate postnatal period. During the first week of life, serum 17a -OHP levels fall ~50-fold as compared to cord blood values. A small transient increase occurs in male infants 30-60 days postnatally. Levels for both sexes remain at constant low levels during childhood, and then progressively increase during puberty reaching adult levels of ~100ng/dl (~3.03nmol/l). As with cortisol, serum 17a -OHP levels normally have an ACTH-dependent diurnal variation, with peak levels in the morning and a nadir at night.
In addition, ovarian production of 17a -OHP increases during the luteal phase of the menstrual cycle. 17-hydroxyprogesterone is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production. Normal levels are 3-90ng/dl in children and in women, 15-70ng/dl prior to ovulation, and 35-290 ng/dl during the luteal phase. Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective, namely 21-hydroxylase and 11ß-hydroxilase, lead to a build-up of 17OHP. In contrast, the rare patient with 17a-hydroxylase deficiency will have very low or undetectable levels of 17OHP. Elevated serum 17 a -OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia.
Catalog #167543
Intended UseCompetitive immunoenzymatic colorimetric method for quantitative determination of 17 OH Progesterone concentration in serum and plasma
Principle of the Test17alpha-OH Progesterone (antigen) in the sample competes with horseradish peroxidase 17alpha-OH Progesterone (enzyme-labelled antigen) for binding onto the limited number of anti-17alpha-OH Progesterone coated on the microplates (solid phase).
After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and theTMB-substrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbances are determined. 17alpha-OH Progesterone concentration in the sample is calculated based on a series by a set of standard. The colour intensity is inversely proportional to the 17alpha-OH Progesterone concentration in the sample.
Detection Range 0-19.2ng/ml
Specificity 100%
Sensitivity 0.1ng/ml
Kit ComponentsMicrotiter Plate, 1x96wells
TMB Substrate, 1x15ml
Stop Solution, 1x15ml
Standard 0, 1x1ml
Standard 1, 1x1ml
Standard 2, 1x1ml
Standard 3, 1x1ml
Standard 4, 1x1ml
Standard 5, 1x1ml
Control, 1x1ml
Conjugate (HRP), 1x6ml
Storage and StabilityStore all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4ºC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.


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