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You are here:Home » Antibodies » Abs to Thioredoxin » Anti -EndoPDI (Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras)

Anti -EndoPDI (Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353;
FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras)

Pricing

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Specifications

Clone Host Grade Applications
Mouse Affinity Purified E B IP IH IF
Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
Catalog #167585
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution1ug/ml was sufficient for detection of EndoPDI in 20ug of heat shocked HeLa cell lysate by Western Blot using Goat anti -mouse IgG:HRP as the secondary antibody.
Western Blot: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type
Concentration~1mg/ml
FormSupplied as a liquid in PBS pH7.4, 50% glyercol and 0.09% sodium azide.
SpecificityRecognizes EndoPDI. Species Crossreactivity: Human. Detects a protein with a mass of ~48kD corresponding to EndoPDI
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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