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You are here:Home » Antibodies » Antibodies-Glucose Transporters » Anti -VGlut1 (BNPI, Slc17a7, Solute Carrier family 17 member 7, Vesicular glutamate transporter 1)

Anti -VGlut1 (BNPI, Slc17a7, Solute Carrier family 17 member 7, Vesicular glutamate
transporter 1)


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Clone Host Grade Applications
Mouse Affinity Purified B IH
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
Catalog #167617
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution1ug/ml was sufficient for detection of VGLut1 in 20ug of rat brain lysate by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type
FormSupplied as a liquid in PBS pH7.4, 50% glycerol.
SpecificityRecognizes rat VGlut1. Species Crossreactivity: Human, Mouse, Rat. Detects ~52kD. No cross-reactivity against VGlut2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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