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You are here:Home » Molecular Biology » Growth Factors-LIF » LIF, Recombinant, Human (Leukemia Inhibitory Factor)

LIF, Recombinant, Human (Leukemia Inhibitory Factor)

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Specifications

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts.
Catalog #167675
SourceRecombinant protein corresponding to aa24-202 from human LIF, expressed in E.coli. (Accession# P15018)
Molecular Weight~19.6kD
Biological ActivityMeasured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells.
The ED50 for this effect is ~0.5ng/ml.
Endotoxin:
<0.10 EU per 1ug of protein by the LAL method
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman LIF, expressed in E.coli. (P15018)
Purity~95% (SDS-PAGE)
Concentration~1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS. No BSA added. Reconstitute with sterile PBS to 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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