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You are here:Home » Antibodies » Antibodies-Proteasomes » Anti -LMP7 (Large Multifunctional Protease 7, PSMB8, RING10, ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i)

Anti -LMP7 (Large Multifunctional Protease 7, PSMB8, RING10, ALDD, D6S216E, JMP, NKJO,
Proteasome Component C13, Proteasome Subunit Y2, PSMB5i)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
PSMB8 (Proteasome Subunit beta type­8; Also b5i, RING10/Y2 and LMP7) is a 23­24kD member of the peptidase T1B family of molecules. It is expressed both constitutively and inducibly by IFN­g in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4+ T cells and monocytes. PSMB8 contributes to the 700kD, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATP­dependent proteolytic activity. PSMB8 qualifies as a b­type, i (immuno)­type proteasome, meaning it both plays a chymotrypsin­like role in the turnover of proteins, and is found in cytokine­responsive cells. The peptides generated through PSMB8 activity are used as immunogens by MHC­I molecules. PSMB8 activity is dependent upon the removal of the PSMB8 precursor prosequence, an action that exposes a critical internal Thr residue. Human PSMB8 is synthesized as a 28­29kD, 276aa proprecursor. It contains a 72aa autocleavable propeptide plus a 204aa mature region. The mature region shows no identifiable standard structural motifs. There is one alternative splice form that shows a 45aa substitution for aa1­49. This isoform does not appear to participate in formation of a proteosome. Over aa73­276, human PSMB8 shares 92%aa sequence identity with mouse PSMB8.
Catalog #167677
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa73­276 from human LMP7/PSMB8 expressed in E.coli (P28062).
SpecificityRecognizes human LMP7/PSMB8. Species sequence homology: Recombinant human PSMB5, 1%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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