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You are here:Home » Antibodies » Abs to Proteasomes » Anti -LMP7 (Large Multifunctional Protease 7, PSMB8, RING10, ALDD, D6S216E, JMP, NKJO, Proteasome Component C13, Proteasome Subunit Y2, PSMB5i)

Anti -LMP7 (Large Multifunctional Protease 7, PSMB8, RING10, ALDD, D6S216E, JMP, NKJO,
Proteasome Component C13, Proteasome Subunit Y2, PSMB5i)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
PSMB8 (Proteasome Subunit beta type­8; Also b5i, RING10/Y2 and LMP7) is a 23­24kD member of the peptidase T1B family of molecules. It is expressed both constitutively and inducibly by IFN­g in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4+ T cells and monocytes. PSMB8 contributes to the 700kD, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATP­dependent proteolytic activity. PSMB8 qualifies as a b­type, i (immuno)­type proteasome, meaning it both plays a chymotrypsin­like role in the turnover of proteins, and is found in cytokine­responsive cells. The peptides generated through PSMB8 activity are used as immunogens by MHC­I molecules. PSMB8 activity is dependent upon the removal of the PSMB8 precursor prosequence, an action that exposes a critical internal Thr residue. Human PSMB8 is synthesized as a 28­29kD, 276aa proprecursor. It contains a 72aa autocleavable propeptide plus a 204aa mature region. The mature region shows no identifiable standard structural motifs. There is one alternative splice form that shows a 45aa substitution for aa1­49. This isoform does not appear to participate in formation of a proteosome. Over aa73­276, human PSMB8 shares 92%aa sequence identity with mouse PSMB8.
Catalog #167677
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa73­276 from human LMP7/PSMB8 expressed in E.coli (P28062).
SpecificityRecognizes human LMP7/PSMB8. Species sequence homology: Recombinant human PSMB5, 1%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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