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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » 2-Phosphoglycerate BioAssay™ Kit (Colorimetric, Fluorometric)

2-Phosphoglycerate BioAssay™ Kit (Colorimetric, Fluorometric)

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Specifications

2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
Catalog #167735
Sample TypeAnimal tissues: e.g. liver, muscle and heart, etc. Cell culture: adherent or suspension cells
Intended UseThe kit is used for measurement of 2-Phosphoglycerate in various tissues/cells, analysis of carbohydrate metabolism, cell signaling and cancer research.
Test PrincipleThe 2-Phosphoglycerate BioAssay™ kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570nm) and fluorescence (Ex/Em = 535/587nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2pg level below 20 pmol and can be used for a variety of sample types.
Kit Components2PG Assay Buffer, 1x25ml
2PG Probe (in DMSO), 1x0.2ml
2PG Enzyme Mix, lyophilized, 1x vial
2PG Converter, lyophilized, 1x vial
2PG Developer, lyophilized, 1x vial
2PG Standard, lyophilized, 1x vial
Storage and StabilityStore powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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