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You are here:Home » Molecular Biology » Growth Factors-IGF » IGF-BP1, Recombinant, Human, (IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1)

IGF-BP1, Recombinant, Human, (IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25,
hIGFBP-1, IGFBP-1)

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Specifications

Human IGF-BP1 is a 25.4kD cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 255aa residues with a N-terminal His-tag.
Catalog #167877
SourceRecombinant corresponding to human IGF-BP1 expressed in Nso cells.
Molecular Weight~28kD
Biological ActivityThe ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100ng/ml.
Endotoxin~0.1ng/ul
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~28kD
SourceNso
Purity~98% (HPLC, SDS-PAGE)
FormSupplied as a lyophilized powder in 10mM HCl. Reconstitute with dH2O to 0.1-1.0mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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