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You are here:Home » Molecular Biology » Growth Factors-IGF » IGF-I, Long R3, Recombinant, Human, (Insulin-like Growth Factor-I, Somatamedin C, IGF-IA)

IGF-I, Long R3, Recombinant, Human, (Insulin-like Growth Factor-I, Somatamedin C, IGF-IA)

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Specifications

IGF-I (insulin-like Growth Factor-I) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Recombinant human IGF-I is a N-terminus modified IGF-I with a Long R3 chain, which shows higher potency than regular human IGF-I in bioactivity assay. The recombinant IGF-I Long R3 was produced from E. coli using Animal Origin Free (AOF) components and therefore it is suitable for bioproduction also. The product can be provided as lyophilized form or liquid form, stable for at least two years.
Catalog #167879
SourceRecombinant corresponding to human IGF Long R3 expressed in E.coli.
Molecular Weight~9kD
Biological ActivityThe ED50 as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6ng/ml.
Endotoxin~0.1ng/ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~9kD
SourceE.coli
Purity~97% (HPLC, SDS-PAGE)
FormSupplied as lyophilized solid. Reconstitute with dH2O or 10mM HAC to 1.0mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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