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You are here:Home » Growth Factors, Cytokines » Growth Factors-NGF » NGF-beta, Recombinant, Human, (Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta, rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF)

NGF-beta, Recombinant, Human, (Human NGF-beta, NGF-beta, NGFbeta, NGF beta, h-NGF-beta,
rh-NGF-beta, recombinant human NGF-beta, recombinant NGF-beta, NGF)

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Specifications

Nerve growth factor-b (NGF-b) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-b is a dimer, formed by two identical 119aa subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119aa and having a molecular weight of 16.95kD. The protein is purified by proprietary chromatographic techniques.
Catalog #167921
SourceRecombinant corresponding to human NGF-beta expressed in CHO cells.
Molecular Weight-17kD
Biological ActivityThe ED50 as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5ng/ml.
Endotoxin~0.1ng/ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17kD
SourceCHO cells
Purity~98% (HPLC, SDS-PAGE)
FormSupplied as a lyophilized powder in 30mM sodium acetate, pH 5.5, 1% HAS. Reconstitute in dH2O to 0.1-1.0mg/ml after centrifugation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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