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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin)

Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IF
In vertebrates 3 main groups of actin isoforms, a, b and g have been identified. A-actins are found in muscle tissues and are a major constituent of the contractile apparatus. B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. B-actins are highly conserved proteins that are involved in cell motility, structure and integrity. B-actins are cytoplasmic proteins. Actin is a loading control and is critical for the correct interpretation of your western blot. B-Actin Loading Control is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Catalog #168005
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:500-1:2000
ELISA: 1:10,000-1:40,000
Western Blot: 1:1000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to aa359-368 from human B-actin.
SpecificityRecognizes human B-Actin. Species Crossreactivity: Mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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