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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin) (Biotin)

Anti -ACTB (Actin, Cytoplasmic 1, Beta-actin) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
In vertebrates 3 main groups of actin isoforms, A, B and G have been identified. The A-actins are found in muscle tissues and are a major constituent of the contractile apparatus. The B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. B-actins are highly conserved proteins that are involved in cell motility, structure and integrity. B-actins are cytoplasmic proteins.
Catalog #168006
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa359-368 from human B-actin.
SpecificityRecognizes human B-actin. Species Crossreactivity: mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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