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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -ARF-BP1 (ARF binding protein 1, HECT, UBA and WWE domain containing 1, HUWE1, Mcl-1 ubiquitin ligase E3, Mule, E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1)

Anti -ARF-BP1 (ARF binding protein 1, HECT, UBA and WWE domain containing 1, HUWE1, Mcl-1
ubiquitin ligase E3, Mule, E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Catalog #168033
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence: 5ug/ml
ELISA: 1:10,000-1:40,000
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to human ARF-BP1 at C-terminus.
SpecificityRecognizes human ARF-BP1. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesARF-BP1, ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule; E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1


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