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You are here:Home » Antibodies » Abs to Nucleotides » Anti -BrdU (Bromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU)

Anti -BrdU (Bromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH FC IF
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Specific for BrdU subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Catalog #168062
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500
ELISA: 1:2000-1:10,000
Flow Cytometry: 1:50-1:100
Immunohistochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
PurityPurified by immunoaffinity chromatography.
ImmunogenBromodeoxyUridine conjugated to KLH
SpecificityRecognizes BrdU.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBromodeoxyuridine 5-bromo-2'-deoxyuridine, BrdU


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