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You are here:Home » Antibodies » Abs to Infectious Disease EBV » Anti -EBV Early Antigens (EBNA1, EBV nuclear antigen 1, Epstein Barr nuclear antigen 1, Human Herpesvirus 4 (HHV4)) (HRP)

Anti -EBV Early Antigens (EBNA1, EBV nuclear antigen 1, Epstein Barr nuclear antigen 1,
Human Herpesvirus 4 (HHV4)) (HRP)


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Clone Host Grade Applications
Monoclonal Mouse Purified B IH IF
Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness. The EBV-induced early antigens (Ea) are among several antigen complexes that have been identified in EBV-infected cells. The Ea complex is composed of diffuse (Ea-D) and restricted (Ea-R) components. The activity of Ea-D is suppressed during latent infection. BMRF1, the gene that encodes for Ea-D, is closely associated with the gene encoding for EBV DNA polymerase, and Ea-D is essential for the activity of this polymerase. Ea-D forms a complex with EBV DNase and, together, they may play a role in viral replication.
Catalog #168764-HRP
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 0.5-1ug/ml
Western Blot: 0.5-1.0ug/ml
Immunoprecipitation: 0.5-1ug/500ug protein lysate
Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Positive ControlEBV-EBV-infected cells or tissues
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
Clone No1108-1
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to horseradish peroxidase (HRP).
PurityPurified by Protein A/G affinity chromatography.
ImmunogenAffinity purified early antigen polypeptides from induced Raji cells precipitated with African Burkitt’s lymphoma sera. Cellular Localization: Nuclear
SpecificityRecognizes EBV.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(EBNA1, EBV nuclear antigen 1, Epstein Barr nuclear antigen 1, Human Herpesvirus 4 (HHV4))

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