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You are here:Home » Antibodies » Abs to Immunoglobulins » Anti -IgG (Immunoglobulin Gamma Heavy Chain, G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4) (Biotin)

Anti -IgG (Immunoglobulin Gamma Heavy Chain, G1m Marker, G2m Marker, G3m Marker, G4m
Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C
Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IH FC IF
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Catalog #168802-Biotin
Applications 
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: For coating, order Ab without BSA
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 0.5-1ug/500ug protein lysate
Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control293T, Raji or hPBL cells. Tonsil or Spleen.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone NoIG266
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to Biotin.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenPurified human Ig Gamma Chain. Cellular Localization: Cytoplasm, Cell Surface and Secreted
SpecificityRecognizes human IgG (Immunoglobulin Gamma Heavy Chain).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(Immunoglobulin Gamma Heavy Chain, G1m Marker, G2m Marker, G3m Marker, G4m Marker, HDC, Heavy Chain Disease Protein, Human Immunglobulin G, Ig gamma1/2/3/4 Chain C Region, IGHG1, IGHG2, IGHG3, IGHG4, Immunoglobulin Heavy Constant 1/2/3/4)


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