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You are here:Home » Antibodies » Antibodies-IgG Kappa » Anti -Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)

Anti -Kappa Light Chain (HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Catalog #168814
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells
Immunohistochemistry: Frozen: 0.5-1.0ug/ml for 30 min at RT
Optimal dilutions to be determined by the researcher.Optimal dilutions to be determined by the researcher.
Positive Control293T, Raji or hPBL cells. Tonsil or Spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone NoTB28-2
FormSupplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 168814AF.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman IgG-kappa myeloma protein. Cellular Localization: Cell Surface, cytoplasmic and secreted
SpecificityRecognizes human Kappa Light Chain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names(HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM)

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