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You are here:Home » Molecular Biology » Growth Factors-FGF » FGF1, Recombinant, Human (Fibroblast Growth Factor 1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1)

FGF1, Recombinant, Human (Fibroblast Growth Factor 1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB,
FGF-alpha, FGFA, GLIO703, HBGF1)

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Specifications

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described.
Catalog #169432
SourceRecombinant protein corresponding to human FGF1 expressed in E. coli (Accession# P05230).
Molecular Weight~15.8kD
Endotoxin<0.1ng/ug
Biological ActivityThe ED50 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <10ng/ml.
Specific Activity>1x105units/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~15.8kD
SourceE. coli
Purity~98% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder in 0.1M NaCl, 10mM Tris, pH 7.6. Reconstitute in 10mM Tris.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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