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You are here:Home » Molecular Biology » Growth Factors-FGF » FGF2, Recombinant, Human (Fibroblast Growth Factor, BFGF, FGFB, HBGF-2)

FGF2, Recombinant, Human (Fibroblast Growth Factor, BFGF, FGFB, HBGF-2)

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Specifications

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Catalog #169440
SourceRecombinant protein corresponding to human FGF2 expressed in E. coli (Accession# P09038).
Molecular Weight~17kD
Endotoxin<0.1ng/ug (1EU/ug)
Biological ActivityThe ED50 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors.
Specific Activity 2x106units/mg.
AA SequenceMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17kD
SourceE. coli
Purity~96% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder in 1MNaCl, 20mM PBS pH 7.0. Reconstitute in ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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