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You are here:Home » Molecular Biology » Growth Factors-FGF » FGF2, Recombinant, Rat, aa10-154 (Fibroblast Growth Factor 2, Fgf-2, bFGF)

FGF2, Recombinant, Rat, aa10-154 (Fibroblast Growth Factor 2, Fgf-2, bFGF)

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Specifications

Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4.
Catalog #169444
SourceRecombinant protein corresponding to aa10-154 from rat Fgf2 expressed in E. coli (Accession# P13109).
Molecular Weight~16kD
Endotoxin<0.1ng/ug (1EU/ug) (LAL gel clot method)
Biological ActivityThe ED(50) determined by dose-dependent stimulation of thymidine uptake by 3T3 cells expressing FGF receptors, was found to be <1ng/ml.
AA SequencePALPEDGGGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~16kD
SourceE. coli
Purity~96% (SDS-PAGE and HPLC)
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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