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You are here:Home » Molecular Biology » Growth Factors-TNF » TNF-alpha, Recombinant, Human (Tumor Necrosis Factor, Cachectin, TNF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNFA, TNFSF2)

TNF-alpha, Recombinant, Human (Tumor Necrosis Factor, Cachectin, TNF, Tumor Necrosis
Factor Ligand Superfamily Member 2, TNF-a, TNFA, TNFSF2)

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Specifications

Tumor Necrosis Factor-alpha (TNF-a) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Catalog #169759
SourceRecombinant protein corresponding to 157aa from human TNF-alpha expressed in E. coli.
Molecular Weight~17.4kD
Biological ActivityThe ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.05ng/ml, corresponding to a specific activity of > 2x10e7 units/mg.
Reconstitution InstructionsReconstitute in water to a concentration of 0.1-1mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~17.4kD
SourceE. coli
Purity~98% (HLPC, SDS-PAGE)
Concentration~0.1-1mg/ml after reconstitution
FormSupplied as a lyophilized powder from 3mM Tris, pH 8.0. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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