Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Osteopontin » Anti -Osteopontin (OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin)

Anti -Osteopontin (OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein
1, SPP-1, Urinary stone protein, Uropontin)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications. OPN is cleaved by members of the matrix metalloproteinase family (MMP) which results in the generation of N-terminal and C-terminal OPN fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.
Catalog #170029
ApplicationsSuitable for use in ELISA (sandwich and indirect). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No14e9
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.15M PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenDSVVYG conjugated to KLH
SpecificityRecognizes native human OPN from purified breast milk. Specific for the MMP cleavage site DSVVYG of human OPN.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links