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You are here:Home » Molecular Biology » MB-Neurotrophins » ProNT-3, Recombinant, Human (Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor)

ProNT-3, Recombinant, Human (Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2,
Neurotrophic factor)

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Specifications

Proneurotrophin-3 (ProNT-3) is the precursor form of neurotrophin 3. ProNT3 functions via p75NTR and sortilin and induces neuronal apoptosis. On the contrary, mature NT3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. As axon retrograde transport is crucial for neurotrophin signaling, Pro-NT3 also plays an important role in the mechanism of retrograde signaling. ProNT3 interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-NT3 plays an important role in retrograde signaling mechanism.
Catalog #170057
SourceRecombinant protein corresponding to human ProNT-3, expressed in E. coli.
Molecular Weight~27.4kD
Endotoxin<0.1ng/ug (1IEU/ug)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~27.4kD
SourceE. coli
Purity~95% (SDS-PAGE, HPLC)
Concentration~1mg/ml after reconstitution
FormSupplied as a lyophilized powder in 20mM PB, pH 7.2, 250mM NaCl. Reconstitute with 1x PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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