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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Cytokeratin 8 (Keratin K8)

Anti -Cytokeratin 8 (Keratin K8)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cytokeratin 8 (Keratin K8), a marker to discriminate simple epithelia from those of different origin. KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation.
Catalog #171531
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Paraffin): 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No14D54
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA 0.09% sodium azide. Reconstitute with1ml sterile dH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytoskeletal proteins from HeLa cells.
SpecificityRecognizes human Cytokeratin 8. Species Crossreactivity: Mouse, rat, tupaia, hamster. Does not recognize bovine and pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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