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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 10 (BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10)

Anti -Cytokeratin 10 (BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IP IH FC IF
This MAb recognizes a protein of 56.5kD, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Catalog #172072
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 0.5-1ug/ml
Western Blot: 0.25-0.5ug/ml
Immunoprecipitation: 0.5-1µg/500ug protein lysate
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Positive ControlA431, HeLa, MCF7 cells or Esophagus.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone NoSPM261
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172072-AF.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenSkin extract of a human Psoriasis patient. Cellular Localization: Cytoplasmic
SpecificityRecognizes Cytokeratin 10. Species Crossreactivity: Human, Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10


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