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You are here:Home » Antibodies » Abs to Mucin » Anti -MUC1 (EMA, CD227, Breast carcinoma-Associated antigen DF3, CA15-3, Carcinoma-Associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface Asso

Anti -MUC1 (EMA, CD227, Breast carcinoma-Associated antigen DF3, CA15-3,
Carcinoma-Associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6,
MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell
surface Asso

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse E B IP IH FC IF
In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Catalog #172127-AF
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT
(No special pretreatment is required for staining of formalin/paraffin tissues)
Optimal dilution for a specific application should be determined.Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Positive ControlMCF-7 or MDA-231 cells. Breast or colon carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoSPM132
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS. Also available with BSA and azide, see 172127.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman milk fat globule membranes. Cellular Localization: Cytoplasmic and cell surface
SpecificityRecognizes human MUC1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEMA, CD227, Breast carcinoma-Associated antigen DF3, CA15-3, Carcinoma-Associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface Associated


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