Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament (NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD, Neurofilament Triplet H Protein, NF-H, NF200, NF-H) (HRP)

Anti -Neurofilament (NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD,
Neurofilament Triplet H Protein, NF-H, NF200, NF-H) (HRP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
This MAb reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Catalog #172132-HRP
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Positive ControlBrain, Neuroblastoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoSPM563
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Conjugated to horseradish peroxidase (HRP).
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman recombinant neurofilament protein. Cellular Localization: Cytoplasmic
SpecificityRecognizes Neurofilament. Species Crossreactivity: Human, Mouse, Rat, Porcine and Chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesNEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD, Neurofilament Triplet H Protein, NF-H, NF200, NF-H


External Links