RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1.
Applications
Suitable for use in Peptide ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000 Western Blot: 0.01-0.03ug/ml Immunohistochemistry: 3-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to CLPNLDHVGHPTER, from the C-terminus of the protein sequence according to NP_000389.1; NP_001123291.1; NP_001165131.1.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CYB5R3. This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).