ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009
Applications
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Immunoprecipitation: 1:20-1:50 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human ANO2.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes ANO2. Species Crossreactivity: human, mouse, rat
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