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210380 Rabbit Anti-CYP2E1 (Cytochrome P450 Enzyme 2E1, CPE1, CYP2E, P450-J, P450C2E)

Specifications
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P05181
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
1571
Gene #
CYP2E1
Shipping Temp
Blue Ice
Storage Temp
-20°C

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Applications
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:20-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human CYP2E1.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes CYP2E1. Species Crossreactivity: human, mouse, rat
Conjugates
Pricing
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