EZH1

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

Q92800

Isotype

IgG

Grade

Affinity Purified

Applications

IF WB

Crossreactivity

Hu Mo Rt

Gene ID

2145

Shipping Temp

Blue Ice

Storage Temp

-20°C

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008).

Applications

Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 1:500-1:2000 Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to human EZH1.

Form

Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

Purity

Purified by affinity chromatography.

Specificity

Recognizes EZH1. Species Crossreactivity: human, mouse, rat

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210764 Rabbit Anti-EZH1 (Enhancer of Zeste Homolog 1, Histone-lysine N-methyltransferase EZH1, KMT6B)

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