Involved in the metabolism of oxesterols, a potent intermediate.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: Neat Western Blot: 1:2 Immunohistochemistry: Neat. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 Optimal dilutions to be determined by the researcher.
Positive Control
Immunohistochemistry: formalin-fixed, paraffin-embedded human liver sections Western Blot: pooled human liver microsomes
Hybridoma
Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ovalbumin-conjugated synthetic peptide CRIEYKQRI (C-terminal sequence)
Form
Supplied as a liquid.
Specificity
Recognizes human Cytochrome P450 39A1.
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