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219056 Rabbit Anti-LIMS1 (LIM And Senescent Cell Antigen Like Domains Protein 1, PINCH, PINCH1, PINCH-1)

Specifications
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P48059
Isotype
IgG
Grade
Affinity Purified
Applications
IF WB
Crossreactivity
Hu Mo Rt
Gene ID
3987
Shipping Temp
Blue Ice
Storage Temp
-20°C

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:50-1:100 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human LIMS1.
Form
Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human LIMS1. Species Crossreactivity: Mouse, rat
Conjugates
Pricing
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