Glypican-4

Clone Type

Polyclonal

Host

Rabbit

Swiss Prot

O75487

Isotype

IgG

Grade

Serum

Applications

FC IHC WB

Crossreactivity

Hu Mo

Gene ID

2239

Gene #

GPC4

Shipping Temp

Blue Ice

Storage Temp

-20°C

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity)

Applications

Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:1000 Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Synthetic peptide corresponding to aa509-520, FEFDYNATDHAG, of glypican-4, conjugated to KLH.

Form

Supplied as a lyophilized powder. Reconstitute with 20ul or 100ul sterile ddH2O.

Purity

Serum

Specificity

Recognizes Glypican-4. Species Crossreactivity: human and mouse

References

1. Siebertz B, Drzeniek Z, Stöcker G, Just U, Handt S, Haubeck HD (1999). Expression of glypican-4 in haematopoietic progenitor and bone marrow stromal cells. Biochem J 344: 937-43.