Tubulin Cleaved by Caspase-6

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

Q71U36

Isotype

IgG

Grade

Ascites

Applications

IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Tubulin is the major constituent of microtubules. Microtubules are assembled from dimers of a- and b-tubulin. a-tubulin cleaved by Caspase-6 can be found in neurons, neurofibrillary tangles, neuropil threads, and neuritic plaques in Alzheimer’s disease.

Applications

Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000 Immunohistochemistry: 1:1000 Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

EEVGVD peptide sequence

Form

Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.

Purity

Ascites

Specificity

Recognizes human Tubulin cleaved by caspase-6.

References

1. Klaiman G, Petzke TL, Hammond J, Leblanc AC. Targets of caspase-6 activity in human neurons and Alzheimer’s disease. Mol Cell Proteomics. 2008 Aug;7(8):1541-55. Epub 2008 May 16.