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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH FC IF

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-kB gene expression. A20 inhibits NF-kB dependent gene expression by interfering with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IkB kinase pathway. A20 is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.
Catalog #	A0000-10
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Flow Cytometry: Intracellular
	Western Blot: 2-4ug/ml
	Immunoprecipitation: 1-2ug/ml
	Immunohistochemistry: Paraffin
	Optimal dilutions to be determined by the researcher.
Positive Control	Jurkat cells, THP1
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	4H16
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant fusion protein of full length human A20 Zinc Finger Protein. Epitope: C-terminal portion of A20 (aa440-790).
Specificity	Recognizes human A20 Zinc Finger Protein.
Species Crossreactivity	Mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.