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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH FC

Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.
Catalog #	A0000-33
Applications	Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 HT-29 cells in a reaction volume not exceeding 200ul.
	ELISA: 0.5-1.0ug/ml; the detection limit is ~0.2ng/well.
	Western Blot: 0.1-0.2ug/ml; The detection limit is ~2ng/lane under non-reducing and reducing conditions.
	Immunohistochemistry: 2-15ug/ml detects A33 in cells and tissues.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.2mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified, recombinant human A33, corresponding to the extracellular domain, aa22-235, expressed in NSO cells (Q99795).
Specificity	Recognizes human A33. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Crossreacts with recombinant mouse A33 ~10%. Species sequence homology: canine 80%, bovine 74%, mouse 71%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.