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You are here:Home » Antibodies » Abs to Proteins » Anti -A33 (GPA33)

Anti -A33 (GPA33)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH FC
Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.
Catalog #A0000-33
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 HT-29 cells in a reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml; the detection limit is ~0.2ng/well.
Western Blot: 0.1-0.2ug/ml; The detection limit is ~2ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 2-15ug/ml detects A33 in cells and tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography
ImmunogenPurified, recombinant protein corresponding aa22-235, extracellular domain, of human A33 expressed in NSO cells (Q99795). Species Sequence Homology: canine; 80%, bovine; 74%, mouse; 71%.
SpecificityRecognizes human A33. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Crossreacts with recombinant mouse A33 ~10%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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