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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A33, MGC129986, MGC129987)

Anti -A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor,
Glycoprotein A33, MGC129986, MGC129987)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH FC
Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214aa in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Catalog #A0000-33B
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug stains 10e6 cells from the HT-29 human colon carcinoma cell line.
Western Blot: 1ug/ml
Immunohistochemistry: 8-25ug/ml stains immersion fixed paraffin-embedded sectionf of human colon and human colon cancer tissue subjected to antigen retrieval, basic.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9H7
HostRat
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa22-235 of human A33 expressed in NS0 cells (Q99795).
SpecificityRecognizes human A33. Does not crossreact with recombinant mouse A33.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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