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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)

Anti -A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor,
MGC129986, MGC129987) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B FC
Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Catalog #A0000-33C
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml
Flow Cytometry: 50ug/ml. Use 10ul of diluted solution/1-2.510e5 cells in <200ul total volume.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS with BSA. Labeled with Biotin. Reconstitute with 1ml TBS, pH 7.3, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
Immunogen
Recombinant corresponding to aa22-235 of human A33 extracellular domain expressed inNSO cells (Q99795).
SpecificityRecognizes human A33. Species crossreactivity: ~10% crossreactivity with recombinant mouse A33.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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