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Anti -A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)
Anti -A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor,
MGC129986, MGC129987) (Biotin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B FC |
|
| Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. | | | Catalog # | A0000-33C | | Applications | Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml | | Flow Cytometry: 50ug/ml. Use 10ul of diluted solution/1-2.510e5 cells in <200ul total volume. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder from PBS with BSA. Labeled with Biotin. Reconstitute with 1ml TBS, pH 7.3, 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | | | | Immunogen | | | Recombinant corresponding to aa22-235 of human A33 extracellular domain expressed inNSO cells (Q99795). | | Specificity | Recognizes human A33. Species crossreactivity: ~10% crossreactivity with recombinant mouse A33. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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