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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AAK1, CT (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048)

Anti -AAK1, CT (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.
Catalog #A0001-95B1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenSynthetic peptide selected from the C-terminal region of human AAK1 (KLH).
SpecificityRecognizes human AAK1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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