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Anti -AAK1 (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048)
Anti -AAK1 (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| AP2-associated protein kinase 1 (AAK1) is a 100kD intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain aa46-315 and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145kD long isoform that contains an additional clathrin-binding domain. Within aa704-822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1. | | | Catalog # | A0001-95G | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 12K1 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa704-822 from human AAK1 expressed in E.coli. | | Specificity | Recognizes human AAK1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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