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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » AatII

AatII

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Specifications

Catalog #A0002
Sequence5'-G A C G T^C-3'
3'-C^T G C A G-5'
Supplied WithRestriction Enzyme Buffer A (R1625): (for 100% AatII digestion) 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with AatII
(10u/ug lambda DNA x 16 hours).
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with AatII more than 95% of the digested DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these sites can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration
10u/ul
Source
Acetobacter aceti
Methylation EffectsBlocked by CG methylation.
Stability during Prolonged IncubationA minimum of 0.3 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNA
A minimum of 5 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
Tail
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer10/13/09- Changed foreign price- 276-JE
Number of Recognition Sites in DNA
Lambda10
PhiX1741
M13mp18/190
pBR3221
pUC18/191
pUC571
pTZ19R/U0
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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